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SUNDAY, SEPTEMBER 29

2:00 PM

Optional Workshop: Basics of Cell Culturing
Gloria Perez, Embryotech Laboratories (Meet in Loeb 306)

5:00 PM

Welcoming Dinner, Third floor of Loeb

7:00 PM

EEO Information and Logistics
Biosafety, Regulatory Restrictions, Student Surveys & Team Assignments
Warren Vieira, Magee Women’s Research Institute

7:15 PM

Brief Overview of Stem Cells, Directed Differentiation and iPSCs
Warren Vieira, Magee Women’s Research Institute.

8:00 PM

Introduction to Stem Cells and Regeneration in Planarian Flatworms
Labib Rouhana, University of Boston, Massachusetts

MONDAY, SEPTEMBER 29

9:00 AM

MBL Welcome
Linda Hyman, MBL, Burroughs Wellcome Director of Education

9:15 AM

Skin Regeneration
Jeff Biernaskie, University of Calgary

10:00 AM

Pre-Lab Prep: hESC Differentiation
Instructor: Diane Carlisle; TA: Mahesh Basantani, University of Pittsburgh
Students will differentiate hESC into their cell lineage of choice. On Day 1, trainees will begin differentiation of their own dish of human iPSCs as well as propagate a plate of iPSCs. Medium will need to be changed on student’s own time on Tuesday, Wednesday, and Thursday following instructions in the lab binder.

10:45 AM

Break

11:00 AM

Pre-Lab Prep: hESC Differentiation
Instructor: Diane Carlisle; TA: Mahesh Basantani, University of Pittsburgh

Noon

Founder’s Luncheon (Loeb 3^rd floor - Fish Bowl)
With Jerry Schatten, University of Pittsburgh, and Jennifer Morgan, MBL (Group 1)

1:00 PM

Lab 1: Deriving Mouse Embryonic Stem Cells by Immunosurgery
Instructor: Cal Simerly, Magee Women’s Research Institute; TA: Emily Robertson, University of Oxford
Students will learn how to isolate the inner cell mouse cells (ICM) from expanded blastocysts using the antibody: complement technique and transfer isolated ICMs for deriving mouse embryonic stem cells.

Lab 2: Regeneration in Planarians
Instructor: Labib Rouhana; TA: Roger Huynh, University of Boston, Massachusetts
Students will observe regeneration in living planarians, visualize neoblast (stem cell) proliferation in regenerating fragments, and then design experiments to test planarians' regenerative capacity and the response to injury.

6:00 PM

Dinner

7:00 PM

Course Participant Introductions

TUESDAY, OCTOBER 1

9:00 AM

Lecture: Using the Mexican Axolotl to Unlock the Secrets of Limb Regeneration
Kate McCusker, University of Massachusetts - Boston

9:45 AM

Lecture: The Mexican Axolotl and Regeneration
James Monaghan, Northeastern University

10:30 AM

Break

10:45 AM

Lecture: Bottom-Up Islet Engineering
Quinn Peterson, Mayo Clinic

Noon

Founder’s Luncheon (Loeb 3rd floor - Fish Bowl)
With Jerry Schatten, University of Pittsburgh, and Jennifer Morgan, MBL (Group 2)

1:15PM

Lab 1: Regeneration in Salamanders: limb regeneration
Instructors: Kate McCusker, University of Massachusetts, Boston; James Monaghan, Northeastern University
Students will use the Accessory Limb Model to study cell cycle dynamics.

Lab 2: Pancreatic Islet Engineering
Instructor: Quinn Peterson, TA: Lauren Jennings, Mayo Clinic
Students will take pluripotent stem cell derived islet spheroids, disperse, and reaggregate them into designer endocrine spheroids.

6:00 PM

Dinner

7:00 PM

Pluripotent Stem Cells and Interspecies Organogenesis
Jun Wu, UT-Southwestern

WEDNESDAY, OCTOBER 2

9:00 AM

10x Presentation

9:45 AM

Break

10:15 AM

Lecture: Reenergizing Regeneration – Cell Metabolism at the Crossroads of Mammalian Digit Regeneration
Mimi Sammarco, Mayo Clinic

11:00 AM

10x Presentation Lecture: Spatial Transcriptomic
Robert Tower, University of Texas - Southwestern

Noon

Founder’s Luncheon (Loeb 3rd floor - Fish Bowl)
With Jerry Schatten, University of Pittsburgh, and Jennifer Morgan, MBL (Group 3)

1:00 PM

Lab: Spatial RNA sequencing
Instructors: Mimi Sammarco, Mayo Clinic; Robert Tower, University of Texas - Southwestern; 10X Representatives

6:00 PM

Dinner

7:00 PM

Stem Cell and Regeneration Research Funding
Ravi Ravindranath, NIH/NICHD

8:00 PM

Career Mentoring Discussion
Q&A panel facilitated by Warren Vieira, and other course faculty.

THURSDAY, OCTOBER 3

9:00 AM

Lecture: Large Animal Models for Germ Cell Research
Ina Dobrinski, University of Calgary

9:45 AM

Break

10:00 AM

Lecture: Derivation of Functional Spermatids from Pluripotent Stem Cells
Chas Easley, University of Georgia

11:30 AM

Free Afternoon to Explore Woods Hole and the Vineyard

1:30 PM

Optional Trip on the Gemma

6:00 PM

Dinner

7:00 PM

T-Shirt Nite Celebration
Come to the MBL Club House

FRIDAY, OCTOBER 4

9:00 AM

Lecture: Investigating Mitochondrial Mechanisms of Neurodegeneration using Patient-Specific Neurons
Diane Carlisle, University of Pittsburgh

9:45 AM

Break

10:00 AM

Lecture: Picking your favorite - Cell identification and Selection Strategies in Human Neural Stem Cell Biology
Jan Pruszak, Paracelsus Medical University (PMU), Austria

10:45 AM

MBL Library Tours and Marine Resources Center

Noon

Founder’s Luncheon (Loeb 3rd floor - Fish Bowl)
With Jerry Schatten, University of Pittsburgh, and Jennifer Morgan, MBL (Group 4)

1:00 PM

Lab 1: hESC Pluripotency & Differentiation
Instructor: Diane Carlisle; TA: Mahesh Basantani, University of Pittsburgh
Students will check the pluripotency of hESC using a live cell stain and also determine the quality of our week-long directed differentiation using immunofluorescent staining.

Lab 2: Isolating & Characterizing Neural stem cells using FACS Strategy
Instructor: Jan Pruszak, Paracelsus Medical University (PMU); TA: Gloria Perez, Embryotech Laboratories
In this laboratory, students will learn the techniques for isolating specific PSC-derived subsets of therapeutic and scientific relevance using flow cytometry.

6:00 PM

Dinner

7:00 PM

Zoom Lecture: Bioengineering CNS Morphogenesis for High-Throughput Screening in Regulatory Science and Disease Modeling Applications
Randolph Ashton, University of Wisconsin - Madison

SATURDAY, OCTOBER 5

9:15 AM

BIOETHICS LECTURE:  "Biotech Boundaries:  Ethical Animal Use and Reproductive Technologies"
Margret Riley, University of Virginia

10:00 AM

Coffee Break (Loeb break room, 3rd floor)

10:30 AM

Alumni Lecture: The influence of sperm on early embryo development: key to advancing assisted reproductive technologies
Maria Gracia Gervasi - SCARE 2018 (University of Connecticut)

11:15 AM

Course Photo – Wear your T-shirts!
Meet in Loeb 1st floor Lobby. Photo Location TBA depending on weather

Noon

Lunch

1:00 PM

Lab 1: Finish all ongoing experiments
Instructors: Cal Simerly, Emily Robertson: Check on mESC derivation.
Instructors: Labib Rouhana. Roger Huynh: Evaluate planarian regeneration
Instructors: Kate McCusker, James Monaghan: Image blastemas.
Instructor: Mimi Sammarco, Robert Tower. Bioinformatic analysis

3:00 PM

Lab 2: Finish all ongoing experiments
Instructors: Quinn Peterson, Lauren Jennings: Students will take pluripotent stem cell derived islet spheroids, disperse, and reaggregate them into designer endocrine spheroids.
Instructor: Mimi Sammarco, Robert Tower. Bioinformatic analysis

4:00 PM

Break

5:00 PM

Pioneer Lecture: What makes a Stem Cell a Stem Cell and How Does it Go Bad?
John Dick, University of Toronto

6:15 PM

Break

6:30 PM

Reception and Awards Banquet
Meigs room (Second Floor Swope)

8:00 PM

Presentation of Pioneer Award and Student Certificates
Ina Dobrinski, Chas Easley

SUNDAY, OCTOBER 6

Check out of rooms before 10AM