2025 AQLM Schedule

This is a draft schedule and will likely be updated.

Time	Event	Faculty	Location
Wednesday, 4/30/25
1:30 - 2:30 PM	Check in and get picture ID
3:00 - 4:00	Welcome and course orientation	Course Directors, Dir. Ed	Speck Auditorium
4:00 - 4:50	LECTURE and LAB: Ray Optics: Making an image and ray tracing	Mattheyses	Loeb 260 (Main Lab)
5:00 - 6:00	Wine and Cheese reception		Tent outside Swope
6:00 - 7:00	DINNER
7:00 - 7:30	LAB Continued	Mattheyses	Main lab
7:30 - 7:50	LECTURE: Ray optics: Apertures and field stops	Mattheyses	Main lab
7:50 - 8:30	LAB: Group work with irises	Mattheyses/ Commercial Faculty	Main lab
8:30 - 8:45	LECTURE: Ray optics: Building a microscope	Mattheyses	Main lab
8:45 - 9:30	LAB: Building a microscope condenser in Kohler illumination; building the imaging path	Mattheyses/ Commercial Faculty	Main lab
9:30 - 9:50	LECTURE: Report out on condenser.��Imaging onto a sensor	Mattheyses	Main lab
9:50 - 10:30	LAB: Building the imaging path; measuring the magnification of your microscope	Mattheyses/ Commercial Faculty	Main lab
Thursday, 5/1/25
8:45 - 8:50 AM	Daily Overview	��	Main lab
8:50-10:30	LECTURE: Wave Optics: Infinity-corrected objectives, Diffraction and Resolution; LAB: Measuring the resolution of your microscope	Mattheyses	Main lab
10:30-11:30	LAB: Kohler on the commercial systems	Mattheyses	Main lab
11:35 - 12:30	Wave optics, diffraction and contrast generation	Kner	Speck
12:30 - 1:30 PM	LUNCH (students need to be there by 1)
1:30 - 2:30	Optics	Ross	Speck
2:30 - 3:30	Image Processing Lecture: FIJI	Wilson	Main lab
3:30-5:30	Image Processing Lab: FIJI	Wilson/Directors	Main lab
5:30 - 6:30	Fourier	Kner	Speck
6:30 - 7:30	Dinner
7:30-10:00	Lightning Talks	Mattheyses	Main lab
Friday, 5/2/25
8:45 - 9:00 AM	Daily Overview	��	Speck
9:00 – 10:00	Label free: Phase/Pol/Darkfield	Kner	Speck
10:00 - 12:00	Label free: Phase/Pol/Darkfield lab	Kner	Main lab
12:00 - 1:00��PM	LUNCH
1:00 - 2:00	Label free: Phase/Pol/Darkfield lab (con't)	��	��
2:00 - 3:00	Abbe demonstration and discussion	��	��
3:00-4:00	PSF/trans/fluor	Laevsky	��
4:00-5:30	Fluorescence Lecture			Salmon	Speck
5:30-6:30	DINNER
6:30-10:00	Fluorescence Lab	Salmon	Main lab
Saturday, 5/3/25
8:45 - 9:00 AM	Daily Overview	��	Speck
9:00 - 10:30**	Digital Detectors	Watkins	Speck
10:30-12:00	Noise lab	Course Directors/Commercial Faculty	Main lab
12:00-1:00	LUNCH
1:00-2:00	Noise Lab con't	Course Directors/ Commercial Faculty	Loeb var
2:00-5:00	Quant lab	Course Directors/ Commercial Faculty	Main lab
5:00-5:30	Noise and Quant discussion	Course Directors	Main lab
5:30-6:30	DINNER
6:30-8:00	Fluorescence Proteins and Probes	Palmer	Speck
8:00-10:00	FRET Biosensors Lab	Palmer	Loeb var
10:00-11:00	Vendor Talks		Main Lab
Sunday, 5/4/25
8:45 - 9:00 AM	Daily Overview	��	Speck
9:00 - 10:00	Quantifying Cell Mechanics	Salaita	Speck
10:00 -10:30	BREAK	��	Main Lab
10:30 -12:00	Live Cell and labeling lecture	Bartle	Main Lab
12:00-1:00 PM	LUNCH
1-5:30	Live Cell and Labeling lab	Bartle	Loeb var
5:30-6	Live Cell Discussion	Bartle	Loeb var
6 - 7:00	DINNER
7:00 - 8:00	3D Microscopy I: The Problems and a Wide-Field Solution	Shaw	Speck
8:00- 11:00	Deconvolution Lab	Shaw	Loeb var
Monday, 5/5/25
8:45 – 9:00 AM	Daily Overview	��	Speck
9:00-10:00	3D Microscopy II: Point-scanning Solutions and Their Problems	Shaw	Speck
10:00-12:30	Confocal Laboratory Exercises	Shaw	Loeb var
12:30-1:30 PM	LUNCH
1:30-2:00	3D Microscopy Discussion I	Shaw	Speck
2:00-3:00	3D Microscopy III: Analysing Performance and Choosing Methods	Shaw	Speck
3:00-5:30	Quantitative Evaluation of Performance in 3D Microscopy	Shaw	Loeb var
5:30-6:30	DINNER
6:30-7:00	3D Microscopy Discussion II	Shaw	Speck
7:00-8:00	TIRF Lecture	Taraska	Speck
8:00-11:00	TIRF Lab	Taraska/Mattheyses	Loeb var
Tuesday, 5/6/25
8:45 – 9:00 AM	Daily Overview	��	Speck
9:00 - 10:00	SR SMM Localization Lecture	Gustavsson	Speck
10:00 - 11:00	SR SMM Single particle tracking lecture	Lidke	Speck
11:00-12:00	SR SIM/STED lecture	Bahlmann	Speck
12:00-1:00	Lunch
1:00-2:00	Vincent A/O demo		��	��
2:00-5:30	SR LAB	Gustavsson, Lidke	Loeb various
5:30-6:30	Dinner
6:30-10:30	SR lab	Gustavsson, Lidke	��
10:30-11:00	SR Discussion	Gustavsson, Lidke	Main Lab
Wednesday, 5/7/25
8:45 - 9:00 AM	Daily Overview	��	Speck
9:00-10:00	LSFM Lecture	Laevsky	Speck
10:00-12:00	LSFM Lab	Laevsky	Loeb various
12:00 - 1:00 PM	Lunch
1:00-3:00	LSFM Lab		Loeb various
3:00-6:00	FREE TIME
6:00-7:00	Dinner
7:00 - 8:00	Advanced Image Analysis Lecture	Cimini / ��ø��	Speck
8:00 - 10:00	Analysis Lab	��ø��	Main lab
10:00-11:00	Ethics	Directors	��
Thursday, 5/8/25
8:45 - 9:00 AM	Daily Overview	��	Main lab
9:00 - 12:00	Machine Learning for Image Processing Laboratory	Cortes	Main lab
12:00 - 1:00 PM	LUNCH
1:00 - 4:00	UC2	UC2	Main lab
4:00 - 5:30	Computer-hardware communication, open source microscope control	UC2	Main lab
5:30 - 6:00	Break
6:00 - 7:00	Inoue Lecture	TBD	Meigs Room
7:00 - 9:00	Clambake	Everyone!	Swope Tent
Friday, 5/9/25
Depart MBL

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